Name AM or PM (Circle one)
Directions: Answer the following in the space provided. Refer to the Safety Regulations and the included Material Safety Data Sheets (MSDS). This assignment is due at the beginning of the next class period.
1) When is it permissible to eat or drink in the laboratory?
2) Is the following statement true? Explain your answer. If the instructor is eating or drinking in the laboratory, then the students have implicit permission do so as well.
3) If someone vomits or cuts him or herself you are to supply only indirect assistance. What does “indirect assistance” mean? Give examples.
4) In our next laboratory exercise we will be working with bacteriologic cultures. What should you do to the lab bench at the beginning and end of the lab exercise?
5) Our next exercise is entitled, “Prokaryotes, Cultures, and Sterile Technique.” What should you do with that exercise before we next meet?
6) What other precautions should you exercise in our next laboratory exercise in regards to your hands, clothing, hair, and eyes?
7) If you or another should have clothing or hair on fire what should be done?
8) Where in the laboratory is the fire blanket kept?
9) Where in the laboratory is the fire extinguisher kept? How does it work and where should one aim the nozzle?
10) What are biohazard containers and what types of biohazard containers are found in the laboratory?
11) Explain how you would dispose of the following items:
a) A broken glass slide with no living specimens on it.
b) An agar petri plate with a bacterial culture growing on it.
c) A cotton swab contaminated with bacteria. The cotton swab is a rigid wooden stick, approximately eight inches long, with a mass of twisted cotton at one end. The wood may penetrate a plastic bag.
d) A broken test tube with blood on it.
e) Animal tissue waste, i.e. the liver from a fetal pig or a preserved cow eye?
f) A paper towel soaked in preservative?
g) The bag that a fetal pig is shipped in after you open it?
12) If you should spill a bacterial culture broth, what should you do?
13) What kinds of things should not be poured down the sink? Give examples.
14) What should you do if you get a chemical or biological hazard in your eyes?
15) Where is the eyewash located in the laboratory?
16) Is the following statement true? Explain. Minor injuries, such as poking oneself with a dissecting needle, do not need to be reported to the instructor.
17) Some preserved materials in the laboratory have been fixed in formaldehyde. Describe acute effects of exposure to high concentrations of formaldehyde.
18) What are acute effects and how are they different from chronic effects?
19) Describe the chronic effects of exposure to formaldehyde.
20) Is a fetus or embryo at risk from exposure to formaldehyde? Explain.
21) How will you protect yourself from exposure to formaldehyde in the laboratory?
22) Most preserved specimens are stored in a product called Carosafe. What is the chemical composition of Carosafe?
23) Is Carosafe safe? Explain.
24) How will you protect yourself from exposure to Carosafe in the laboratory?
25) Briefly summarize the breakage policy.
PALOMAR COLLEGE LIFE SCIENCES DEPARTMENT
SAFETY AGREEMENT and BREAKAGE FEE AGREEMENT
Students are expected to understand and follow the Health and Safety regulations that are presented in this course. Failure to comply with these regulations may result in dismissal from the course.
I have read the Laboratory Safety Rules for this Life Sciences course. I both understand and agree to abide by these rules in the classroom and laboratory at Palomar College.
_________________________________ ___________________
Signature Date
Rules for the disposal of Medical Waste and other laboratory waste are included in my copy of Laboratory Safety Instructions. I both understand and agree to abide by these rules in the laboratory at Palomar College.
_________________________________ ___________________
Signature Date
I have read the Breakage Fee Policy for the Life Sciences Department. I both understand and agree to abide by the Life Sciences Department Breakage Fee Policy.
_________________________________ ___________________
Signature Date
_________________________________ ___________________
Student Name (PRINT) Course
_________________________________ ___________________
SAFETY REGULATIONS
“It's better to be safe a hundred times than be killed once.”
Mark Twain
Safety is always an important component to a laboratory experience. One of the most important aspects of lab safety is advance preparation. Be sure to read the laboratory exercise thoroughly prior to your lab period.
The most significant hazard to your safety in the BIOLOGY 201 Laboratory is the preservative that is used on your dissection specimens. Although manufacturers make every effort to minimize the amount of Formaldehyde in the preservative, it is present. Formaldehyde, in its concentrated form, is HIGHLY TOXIC. Attached to this safety handout are the Material Safety Data Sheets for Formalin and the other preservatives with which you will come into contact in these laboratory exercises. Read these sheets and familiarize yourself with them, particularly the hazards, precautions, and symptoms of overexposure to these preservatives. Wear eye protection when working with preserved specimens. If you have known allergies to any material used in this laboratory, discuss the problem with the instructor.
In BIOLOGY 201 your laboratory exercises will cover a broad range of topics. You will handle chemicals, preserved specimens, and bacterial cultures. The procedures for safe handling of these laboratory materials and the habits you develop during this course will be useful to you in the future, especially if you take other laboratory courses. BE AWARE THAT SUBSTANCES SUCH AS FERTILIZER, THAT MAY BE USED IN YOUR GREENHOUSE LABORATORY EXERCISES, ARE CHEMICALS AND MUST BE USED IN A SAFE MANNER. Keep in mind that any laboratory material may pose a significant threat to your health or safety if misused.
As in driving, a person must be "defensive" in the laboratory. Pay attention at all times during lab, to the activities of your fellow students as well as to your own work.
Do not bring any food or beverages into the laboratory. If you need to eat or drink during a lab period, please wash your hands and then step outside of the room. This will prevent contamination of your food or drink with laboratory materials (chemicals, preservatives, etc.) that may be present in the classroom.
Cuts and burns are the most common injuries that occur in laboratories. Shoes must be worn at all times in a laboratory. Open toed shoes and flip flops should not be worn in a laboratory setting as they create a hazard for cuts from broken glass and burns from chemical spills. Loose clothing and unrestrained hair are a fire hazard.
The potential for exposure to HIV and Hepatitis viruses requires that everyone take precautions to protect themselves from contact with blood and other body fluids. Exposure can occur via open wounds, sores, or any break in the skin including those caused by such things as chapping and dermatitis. In the event of a minor bleeding injury in the laboratory, give absorbent material (paper towel) to the victim and instruct the victim to apply pressure to the wound. Do not offer DIRECT assistance to the victim unless you are properly protected with disposable latex gloves. Likewise, if someone becomes ill (vomits) in a classroom setting, offer INDIRECT assistance. Contact your instructor to arrange for cleanup of body fluids, as special supplies are needed in order to perform the cleanup and disposal safely.
In case of a fire, never put yourself in danger of being burned. Warn others, clear the area immediately, and help other students who may require assistance. If your clothing (or the clothing of others) catches fire, smother the flames by rolling on the floor. Do not wrap a person who is standing in a fire blanket as it can increase the severity of a burn injury by creating a chimney effect.
When working with bacteriologic cultures, special care must be used. Proper use of disinfectants and proper disposal of bacteriologic waste is extremely important, as is proper handling of bacterial spills. When working with bacteria:
1) If you have open wounds or cuts on your hands, cover them with a bandage or wear latex gloves.
2) Disinfect lab bench tops before and after handling bacterial cultures.
3) While handling bacteria, keep your hands away from your eyes, nose, and mouth.
4) Wash your hands thoroughly after handling bacterial
5) If a culture is spilled, cover the spill area with paper towel and flood the paper towels with disinfectant. Wait 20 minutes and then wipe up the spill with more paper towels and dispose of the paper towel in a red biohazard bag.
Disposal of laboratory waste in a proper manner has both legal and financial consequences.
Solid and liquid laboratory waste must be separated by type depending on how it must be treated prior to disposal and how it will be disposed of. There are many types of disposal containers used routinely in the laboratory.
1) The wastebasket: is for paper towel from hand washing, and other non-laboratory, non-contaminated waste. The term “non-contaminated” means free of harmful things, e.g. bacteria and hazardous chemicals. Soda cans, water bottles, and other recyclables should be placed in recycle containers outside of the classroom.
2) The broken glass container is a cardboard box, lined with plastic and is for the disposal of BROKEN laboratory glass that is non-contaminated. Your instructor will arrange for the disposal of broken contaminated glassware. Glass soft drink bottles should be recycled.
3) The "Sharps Container" is made of red puncture-proof plastic and is for the disposal of items that require autoclaving prior to disposal that would puncture the plastic of the biohazard bag. Examples of such items include: syringes with needles and cotton swabs, microscope slides, and disposable pipettes that have been in contact with microorganisms. No other objects should be put in a sharps container. Do not put glassware with hazardous chemicals into a sharps container. Your instructor will arrange for proper disposal of glassware contaminated with hazardous chemicals.
4) The Animal Tissue Disposal container is a covered trash can containing a red bag. It is for disposal of dissected animal tissue that will be disposed of by incineration.
5) The Preservative Disposal container: is a covered trashcan into which are poured the excess liquid contents of the preserved specimen bag. This material is disposed of as a hazardous chemical waste.
6) The Bag Disposal container is a covered trash can for collecting the plastic bags that are contaminated with preservative liquid
7) The Glove and Paper towel Disposal container is a covered trash can used to collect gloves or preservative soaked paper towel.
If there will only be a small amount of preservative liquid, bags, or animal tissue generated in a laboratory exercise, the materials may be collected in one container for separation later by lab personnel. Preservative that is spilled should be immediately wiped up with paper towels, which should then be disposed of in the Glove and Paper towel container.
Report all accidents or injuries to your instructor. We are required to have each student who is injured in a laboratory evaluated by Student Health Services. Avoid direct contact with the preservative. If a preservative contacts your skin, wash immediately with water for at least 5 minutes and inform your instructor. If preservative contacts your eyes, use the eyewash to flush your eyes for at least 20 minutes. Hold your eyelids open. In both cases your instructor must be informed and arrangements made for you to be seen at Student Health Services for evaluation.
Before leaving the lab, make sure that your dissecting table is clean and that your dissecting tray is washed, dried and stored in the appropriate location.
BREAKAGE FEE POLICY - LIFE SCIENCES DEPARTMENT
1) In the event of damage or breakage of supplies and equipment by students of the department the following policy shall apply:
2) This policy will NOT determine whether the breakage was from neglect or by accident. It will apply equally in all cases.
3) This policy will be enforced on all breakage over $5.00 cost of replacement.
4) All Instructors will discuss this policy and information with their students at the beginning of the semester. A sign-off sheet is required as well.
5) When damage or breakage occurs, the Instructor must be notified. At that time the breakage form will be filled out by both the Instructor and the student.
6) The Instructor will submit the top half of the form to the Department Secretary or an Instructional Assistant, to be forwarded to the CASHIERS OFFICE.
7) The bottom half of the form is given to the student. It is the student's responsibility to pay the appropriate amount to the cashier. The Instructor and the department will not remind the student to pay the damage fee.
8) When notified by the department (by receipt of the top half of the breakage form), the CASHIERS OFFICE will note the student's file with a 'monetary hold - fees due'. At this point, the student owes the college money and cannot register for the next semester nor request transcripts until the debt is paid.
9) When the student pays the cashier the proper amount, the 'monetary hold' on the student's file is removed.
